Loss of LCs is due to the LC-intrinsic ablation of p14. (A-B) Analysis of epidermal cells for repopulation of LCs 20 weeks after BM transfer. Reconstitution of CD11c-p14_del mice with BM from LangerinEGFP mice results in a MHC II⁺ LC population consisting mainly of donor EGFP⁺ BM cells (LangerinEGFP→CD11c-p14_del). This is comparable to the control approach, in which BM from control mice was transferred into LC-depleted LangerinDTR mice (Control→LangerinDTR). LangerinEGFP BM transfer into control mice yields an MHC II⁺ LC population of mostly recipient origin, that is, EGFP^neg (LangerinEGFP→Control). One representative mouse for each BM chimera in panel A (n = 2); combined data from 3 individually analyzed mice per group in panel B. (C-D) Analysis of adult Langerin-p14_del and control mice for the presence and maturation status of LCs in whole skin (pre-gated for viable CD11c⁺ cells) and epidermis (gated for viable cells only). Histogram: isotype, gray filled; control, dotted line; Langerin-p14_del, black line. One representative mouse in panel C (n = 3); combined data (corresponding to epidermal cell analysis) from 4 individually analyzed mice per genotype in panel D. *P < .05, **P < .01, ***P < .001.