CD11c-specific depletion of p14 leads to increased LC apoptosis. (A) Epidermal sheets of control and CD11c-p14_del mice were obtained on postnatal days 1, 4, 7 from abdominal skin and on day 15 from ear skin. LCs were stained for MHC II (green fluorescence) and the apoptosis marker active caspase-3 (red fluorescence, arrowheads). Scale bar: 50 µm. (B-C) Enumeration of total and apoptotic LCs at indicated time points after birth. Fifteen pictures of randomly chosen areas were recorded and the total number of MHC II⁺ LCs as well as the number of MHC II and active caspase-3 double-positive apoptotic LCs per mm² was determined. The number of apoptotic LCs on day 1 after birth could not be determined (n.d.) because too few LCs were present. Combined data from 3 individually analyzed mice per genotype and time point are presented. ***P < .001.