CD11c-specific depletion of p14 inhibits mitosis of LCs. (A-B) Quantification of total and mitotic LCs of 7-day-old CD11c-p14_del and control mice. LCs are stained for MHC II (red fluorescence) and phospho-Histone H3 (green fluorescence). Fifteen pictures of randomly chosen areas were recorded and the total number of LCs per mm², as well as the proportions of mitotic LCs (arrowheads), were quantified. Combined data from 3 to 4 mice per genotype are depicted in panel B. Scale bar: 20 µm. (C) Staining patterns of phospho-Histone H3 allow identification of mitotic phases: late G₂ or prophase, metaphase, and telophase/cytokinesis. All stages of mitotic LCs were found both in CD11c-p14_del and in control mice. Examples shown here are from control mice. (D-E) LC cell-cycle distribution based on BrdU/7AAD incorporation on day 8 after birth. MHC II⁺ LCs and CD3⁺ DETCs were analyzed for intercalation of BrdU and 7AAD and further divided into 3 cell-cycle phases: G₁/G₀: BrdU^neg7AAD^low; S: BrdU⁺7AAD^low-high; G₂/M: BrdU^neg7AAD^high. One representative experiment of an 8-day-old neonatal mouse in panel D (n = 5); combined data from 8 individually analyzed mice per genotype in panel E. **P < .01, ***P < .001.