CD11c-specific depletion of p14 leads to the recruitment of short-term LCs but not long-term LCs to inflamed skin. (A-B) Analysis of the total LC population (MHC II⁺ cells in the epidermus) on day 0 (ie, untreated) as well as 7, 21, and 35 days after TNCB treatment. The proportions of short-term LCs (MHC II⁺ langerin^neg cells) and long-term LCs (MHC II⁺ langerin⁺ cells) were determined for each time point. One representative experiment of each genotype and time point is shown in panel A; combined data from at least 4 individually analyzed mice per genotype and time point in panel B. (C) Immunofluorescence microscopy analysis of long-term– and short-term–LCs (arrows) in situ, 7 days after TNCB treatment. LCs were stained for langerin (green fluorescence) and MHC II (red fluorescence). *Hair follicles. One representative mouse of 3 mice per genotype is shown in panel C. Scale bar: 200 µm for lower magnification, 50 µm for higher magnification pictures. *P < .05, **P < .01, ***P < .001. LT-LC, long-term LC; ST-LC, short-term LC.