CD11c-specific depletion of p14 leads to reduced expression of the LAMTOR complex and impairs ERK and mTORC1 signaling. (A) Scheme of BMDC culture, derived from CD11c-p14_del and control mice. (B) Analysis of the percentage of CD11c⁺ BMDCs as well as their tdTomato expression on day 8 of CD11c-p14_del and control BM culture. One representative experiment of 2 is shown. (C,F) Analysis of phosphorylation and subsequent quantification of the MAPK effector kinases ERK1 and 2, JNK, and p38 in CD11c⁺ BMDCs derived from CD11c-p14_del and control BMDCs. (D) Expression analysis of LAMTOR complex proteins: p18, MP1, p14, HPXIP, and C7orf59 in CD11c⁺ BMDCs derived from CD11c-p14_del and control BMDCs. (E-F) Analysis of phosphorylation and subsequent quantification of the mTOR pathway related molecules AKT, mTOR, p70S6K1, and S6 kinase in CD11c⁺ BMDCs, derived from CD11c-p14_del and control BMDCs. One representative experiment of 3 is shown in panels C-E. Combined data from 3 to 4 individually analyzed mice per genotype are depicted in panels F and G. (H) Scheme of LAMTOR complex disruption caused by loss of p14, which leads to malfunction of the ERK and mTOR pathway in p14-deficient DCs. *P < .05, **P < .01, ***P < .001.