Differentially expressed genes are enriched in neutrophil function-related factors and overlap with DNA methylation changes. (A) Lists of differentially expressed genes were generated from microarray analysis based on a BH adjusted P < .05 and a fold change >2 between any 2 cell populations (eg, CMP vs GMP and CMP vs PMC). The lists of up-regulated genes from all comparisons and the lists of down-regulated genes from all the comparisons were combined. Genes appearing on both lists (oscillating genes) were removed and analyzed separately (supplemental Figure 6C). Gene lists were used for gene ontology analysis using the DAVID Bioinformatics Resources. The tables include selected GO terms. Only terms not present in both lists are shown. BH, Benjmini-Hochberg adjusted P value. (B) Venn diagrams showing the overlap between differentially methylated and differentially expressed genes. Lists of differentially methylated genes include all genes with ≥1 DMS from 1500 bp upstream of TSS to the end of the 3′UTR. Separate lists for genes with increased and decreased methylation were made, excluding genes with oscillating DMSs or >1 DMS changing in separate directions. Lists of differentially methylated genes and differentially expressed genes were filtered for genes present on both platforms and compared for overlap. P values were calculated using the hypergeometric distribution test. METdwn, genes with decreased methylation; METup, genes with increased methylation; EXPdwn, genes with decreased expression; EXPup, genes with increased expression; METall, all differentially methylated genes; EXPosci, genes with oscillating expression.