Defect in the recirculation of B and T cells post-reimmunization. (A) Clec1b^fl/flPF4-Cre and Clec1b^fl/fl mice were immunized with 10 μg of NP-CGG precipitated with alum on day 0, day 21, and day 42 in the front paw pad. (B) After 8 days, flow cytometric analysis was performed on the single-cell suspensions of the draining and nondraining brachial LNs of Clec1b^fl/fl control (●) and Clec1b^fl/flPF4-Cre (○) littermates, stained with CD45, CD4, CD19. Absolute numbers of CD45⁺ cells, CD4⁺ T cells, and of CD19⁺ B cells are indicated in the dot plots. Unpaired Student t test, *P < .05, **P < .01.