BH3-domain selectivity can determine antiapoptotic capacity when selective BH3-only proteins Bad or Noxa are exclusively involved. (A-B) J16 cell lines transduced to express the individual prosurvival Bcl-2 proteins as N-terminal GFP fusions and sorted on equal protein levels (as in Figure 5A) were treated with a dose range of ABT-737 (A) or bortezomib (B). Cell death was assessed by PI uptake after 48 hours. Empty vector (E.V.) transduced cells were used as control and treated with QVD-OPH (E.V. Q) to show that cell death was apoptotic. (C) J16 cell line with Dox-inducible expression of tBid-C was transduced to express the individual prosurvival Bcl-2 proteins as N-terminal GFP fusions and the resulting cell lines were sorted on equal protein levels (as in Figure 5A). Cells were treated with the indicated dose range of Dox, and cell death was assessed by PI uptake 48 hours after Dox addition. Data shown are mean values ± SD derived from 3 independent experiments.