NETs form in mouse models of thrombosis and cancer. (A) Intravital microscopy of developing thrombi shows the release of NETs early (3 hours) and more prominently in occlusive thrombi (48 hours). Arrows indicate NETs. Sytox Green, DNA. Scale bars, 50 μm. Reproduced from von Bruhl et al.³⁴  (B) Composite image of a thrombus formed in a WT mouse 48 hours after IVC stenosis. Mosaic generated using MosaicJ plug-in for ImageJ software.¹²⁶  Citrullinated histone H3 (H3Cit) staining (green) shows evidence of a NET meshwork throughout the red portion of the thrombus. Scale bar, 100 μm. (C) Mice bearing a mammary carcinoma develop spontaneous thrombi in the lung (right) after 28 days, a time point when NETs are spontaneously generated in these mice. This does not occur in tumor-free mice (left). VWF, green. Fibrinogen, red. DNA, blue. Scale bar, 50 μm. Reproduced from Demers et al.⁷¹