SATB1 suppression in PCALCL cells induced cell-cycle arrest at the G0/G1 stage. (A) MTS-based cell viability assay demonstrated a lack of increase in viable cells in 2 SATB1-silenced lines, whereas control Mac-1 cells demonstrated a normal growth curve. (B) The size of colonies formed in the CFC assay was decreased in SATB1-suppressed Mac-1 cells compared with control cells. (C) The number of colonies formed in semisolid culture (CFC output) decreased more than 10-fold upon SATB1 suppression. (D-E) Accumulation of the G0/G1 population and decrease of the S and G2/M populations were observed in Mac-1 cells with SATB1 silencing via PI cell-cycle analysis. (F-G) Long-term culture of the transduced cells revealed an increase in the Annexin V⁺ population in SATB1-silenced cells, which indicates increased cell apoptosis. Mac-1-sh1 to Mac-1-sh2, SATB1-silenced Mac-1 cell lines with 2 independent hairpins against SATB1. Mac-1 cells transduced with scrambled shRNA (Mac-1-sh0) and parental cells served as controls. *P < .05. Each experiment was repeated 2 times with 3 biological replicates.