AMG 330 recruits memory T cells for target cell lysis. (A) Continuous lysis of repeatedly added AML blasts by autologous T cells from complete remission (CR) using AMG 330. Black arrows indicate AML blast addition. CD3⁺ T-cell expansion is represented in the graph by columns. (B) Expansion of CD3⁺ T cells from AML patients at primary diagnosis in a co-culture using AMG 330 compared with absence of proliferation with control antibody. One representative experiment (PT2) is shown (n = 14). CD3⁺ T cells are given in absolute numbers/well. (C) FACS analysis of the expanding T-cell population differentiating between CD4⁺ and CD8⁺ T cells and (D) between central memory (CD45RA⁻/CCR7⁺) and naïve (CD45RA⁺/CCR7⁺) T cells. All dot plots are gated on CD3⁺ cells. (E) Percentage of regulatory T cells (CD4⁺/CD25⁺/FoxP3⁺) in the expanding T-cell population after 3 and 9 days of culture. Dot plots are gated on CD3⁺/CD4⁺ T cells. (F) Expression of T cell activation markers Tim-3, LAG3 (n = 1), and PD-1 (n = 3) during co-culture. All histograms are gated on CD3⁺ cells.