![Figure 6. The proliferation and survival defects of mature T cells from p57-deficient mice are not rescued by additional ablation of p53. (A) Number of TCRβ-positive cells among splenocytes from individual Lck-Cre/p57[+]/+, Lck-Cre/p57[+]/F, and Lck-Cre/p57[+]/F/p53−/− mice at 7 weeks of age (n = 3). (B) Splenic CD3+ T cells from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age were stimulated with anti-CD3ε (5 μg/mL) for the indicated times, after which the abundance of p21, Noxa, and Bax mRNAs was determined by RT and real-time PCR analysis. Normalized data are expressed relative to the corresponding value for cells from control mice at time 0 and are means ± SD for 3 mice. (C-D) Splenic CD3+ T cells from Lck-Cre/p57[+]/+, Lck-Cre/p57[+]/F, Lck-Cre/p57[+]/+/p53−/−, and Lck-Cre/p57[+]/F/p53−/− mice at 7 weeks of age were stimulated with anti-CD3ε for 36 hours and exposed to BrdU during the final 1 hour of incubation. They were then stained with anti-BrdU and propidium iodide, and the percentages of BrdU-positive cells (C) and of sub-G1 (apoptotic) cells (D) were determined by flow cytometry. Data are means ± SD for 3 mice. (E) Splenic CD3+ T cells from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age were stimulated as in panel B, after which the abundance of caspase-8 and Bid mRNAs was determined by RT and real-time PCR analysis. Normalized data are expressed relative to the corresponding value for cells from control mice at time 0 and are means ± SD for 3 mice. ***P < .005.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/22/10.1182_blood-2013-10-532390/4/3429f6.jpeg?Expires=1735551425&Signature=DBL~O9N69~M2WvN8NC~j4efAsp5SHpjrrTREa4bOn6zPQsmj8Q~0XQzbnPc4Q6c5C1t~bYngKJdP3WEbsMIGLHASR9Mi98QTN0Kb7GoA9zCT4kNoBgqnNlo9QDFVJJ58cS4QrxvwPJG~JfaWluczKeqMGl8IdJjhQGnBrB8iywMAUIgYuspkrAV40XoOPSd2Cc5Pgbw5Kvcpc10yWp0OkdQIPUHIfJTXrjNJpLBHFxE514Vbt3oaKC2xrWbNkICkXHLVoHNzMc5lG-SVrrryoavz4O-ay1M~8q85QPTjgakYmrqA~d~WDfVcyKnTGa64sKjwABdYMO64gNZ1bxM8Pg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The proliferation and survival defects of mature T cells from p57-deficient mice are not rescued by additional ablation of p53. (A) Number of TCRβ-positive cells among splenocytes from individual Lck-Cre/p57[+]/+, Lck-Cre/p57[+]/F, and Lck-Cre/p57[+]/F/p53−/− mice at 7 weeks of age (n = 3). (B) Splenic CD3+ T cells from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age were stimulated with anti-CD3ε (5 μg/mL) for the indicated times, after which the abundance of p21, Noxa, and Bax mRNAs was determined by RT and real-time PCR analysis. Normalized data are expressed relative to the corresponding value for cells from control mice at time 0 and are means ± SD for 3 mice. (C-D) Splenic CD3+ T cells from Lck-Cre/p57[+]/+, Lck-Cre/p57[+]/F, Lck-Cre/p57[+]/+/p53−/−, and Lck-Cre/p57[+]/F/p53−/− mice at 7 weeks of age were stimulated with anti-CD3ε for 36 hours and exposed to BrdU during the final 1 hour of incubation. They were then stained with anti-BrdU and propidium iodide, and the percentages of BrdU-positive cells (C) and of sub-G1 (apoptotic) cells (D) were determined by flow cytometry. Data are means ± SD for 3 mice. (E) Splenic CD3+ T cells from Lck-Cre/p57[+]/+ and Lck-Cre/p57[+]/F mice at 8 weeks of age were stimulated as in panel B, after which the abundance of caspase-8 and Bid mRNAs was determined by RT and real-time PCR analysis. Normalized data are expressed relative to the corresponding value for cells from control mice at time 0 and are means ± SD for 3 mice. ***P < .005.
