Figure 1
Figure 1. Id2 represses IL-10 expression by virus-specific CD8+ T cells. (A) Weight loss in wild-type or Id2fl/flLckCre+ mice infected intranasally with 104 PFU HKx31 influenza virus. Data show mean weight ± SEM pooled from 2 independent experiments. Statistically significant differences were determined using an unpaired 2-tailed Student t test; *P < .05. (B) Quantification of IL-1β and TNF-α in the bronchoalveolar fluid of mixed bone marrow chimeras in Id2fl/flLckCre+ (Ly5.2+):wild-type (Ly5.1+) → wild-type F1 (Ly5.2+Ly5.1+) or wild-type (Ly5.2+):wild-type (Ly5.1+) → wild-type F1 (Ly5.2+Ly5.1+) 8 days after infection with HKx31 (n = 6 from 2 independent experiments). Statistically significant differences were determined using an unpaired 2-tailed Student t test (TNF-α) or a Mann-Whitney U test (IL-1β); *P < .05. (C) Microarray analysis of DbNP366-specific Id2fl/flLckCre+ and wild-type CD8+ T cells isolated from the spleen of PR8-primed/HKx31-infected mixed bone marrow chimeric mice. Data show the mean fold difference of genes from 3 biologically independent analyses. (D) Quantitative analysis by real-time PCR of Il10 mRNA from wild-type or Id2fl/flLckCre+ DbNP366-specific (KLRG1−) CD8+ T cells from PR8-primed/HKx31-infected mixed bone marrow chimeras (day 9) and naïve CD44−CD62L+ CD8+ splenic T cells. Data show the mean Il10 expression ± SEM relative to Hprt from 3 independent samples. (E-F) Intracellular expression (E) and frequency (F) of IL-10 in IFN-γ+ Id2fl/flLckCre+ (Ly5.2+) or wild-type (Ly5.1+) CD8+ T cells isolated from the spleen and the lung of PR8-primed mixed bone marrow chimeras 9 days after infection with HKx31. Data show the mean ± SEM (spleen, n = 11; lung, n = 9 mice per genotype pooled from 3 independent experiments). (D,F) Statistically significant differences were determined using a 2-tailed paired Student t test; ***P < .001. (G-H) IL-10-GFP expression (G) and frequency (H) in Id2fl/flLckCre+ (Ly5.2+) or wild-type (Ly5.1+) CD8+ and CD4+ T cells from the bronchoalveolar lavage from mixed bone marrow chimeras 9 days after infection with HKx31. (H) Data show the mean ± SEM (n = 6 mice per genotype pooled from 2 independent experiments). Statistically significant differences were determined using a Mann-Whitney U test; **P < .01. WT, wild-type.

Id2 represses IL-10 expression by virus-specific CD8+ T cells. (A) Weight loss in wild-type or Id2fl/flLckCre+ mice infected intranasally with 104 PFU HKx31 influenza virus. Data show mean weight ± SEM pooled from 2 independent experiments. Statistically significant differences were determined using an unpaired 2-tailed Student t test; *P < .05. (B) Quantification of IL-1β and TNF-α in the bronchoalveolar fluid of mixed bone marrow chimeras in Id2fl/flLckCre+ (Ly5.2+):wild-type (Ly5.1+) → wild-type F1 (Ly5.2+Ly5.1+) or wild-type (Ly5.2+):wild-type (Ly5.1+) → wild-type F1 (Ly5.2+Ly5.1+) 8 days after infection with HKx31 (n = 6 from 2 independent experiments). Statistically significant differences were determined using an unpaired 2-tailed Student t test (TNF-α) or a Mann-Whitney U test (IL-1β); *P < .05. (C) Microarray analysis of DbNP366-specific Id2fl/flLckCre+ and wild-type CD8+ T cells isolated from the spleen of PR8-primed/HKx31-infected mixed bone marrow chimeric mice. Data show the mean fold difference of genes from 3 biologically independent analyses. (D) Quantitative analysis by real-time PCR of Il10 mRNA from wild-type or Id2fl/flLckCre+ DbNP366-specific (KLRG1−) CD8+ T cells from PR8-primed/HKx31-infected mixed bone marrow chimeras (day 9) and naïve CD44−CD62L+ CD8+ splenic T cells. Data show the mean Il10 expression ± SEM relative to Hprt from 3 independent samples. (E-F) Intracellular expression (E) and frequency (F) of IL-10 in IFN-γ+Id2fl/flLckCre+ (Ly5.2+) or wild-type (Ly5.1+) CD8+ T cells isolated from the spleen and the lung of PR8-primed mixed bone marrow chimeras 9 days after infection with HKx31. Data show the mean ± SEM (spleen, n = 11; lung, n = 9 mice per genotype pooled from 3 independent experiments). (D,F) Statistically significant differences were determined using a 2-tailed paired Student t test; ***P < .001. (G-H) IL-10-GFP expression (G) and frequency (H) in Id2fl/flLckCre+ (Ly5.2+) or wild-type (Ly5.1+) CD8+ and CD4+ T cells from the bronchoalveolar lavage from mixed bone marrow chimeras 9 days after infection with HKx31. (H) Data show the mean ± SEM (n = 6 mice per genotype pooled from 2 independent experiments). Statistically significant differences were determined using a Mann-Whitney U test; **P < .01. WT, wild-type.

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