Co-expression of CD8, CD45RA, and CCR7 in CAR-T cells correlates with their in vivo expansion in lymphoma patients. (A-B) Illustrates the correlation between the percentage of CD45RA⁺ and CAR⁺CD45RA⁺ cells in the T-cell products and the peaks of the qPCR signals of CAR-T cells in the peripheral blood of infused patients. (C) Detection of CAR-T cells by qPCR in the peripheral blood of patients who received T-cell products with low or high CAR⁺CD45RA⁺ cells, respectively. (D) Equal CAR expression by phenotypic analysis in CD45RA⁺ and CD45RO⁺ cells. (E) Representative flow cytometry plots of CAR-T cells with low and high frequency of CD8⁺CD45RA⁺CCR7⁺ cells. (F) Peaks of the qPCR signals of CAR-T cells in the peripheral blood of patients infused with CAR-T cell lines containing >5% or <5% CAR⁺CD8⁺CD45⁺CCR7⁺ cells.