Figure 3
Figure 3. IL-7 and IL-15 do not promote preferential transduction of naïve T cells. (A) Schematic representation of the labeling process to distinguish T cells originated from naïve (CD45RA+) and antigen-experienced (CD45RO+) T cells by labeling with PKH26 dye before activation and retroviral transduction with either IL-2 or IL-7 and IL-15. (B) Gating strategy and representative data showing CAR expression by T cells originated from naive T cells. (C) CAR expression in PKH26– cells (originally CD45RA+) after activation and transduction (n = 5). (D) Percentage of Ki67+ cells on PKH26– cells (originally CD45RA+) prior to retroviral transduction (n = 5). (E-F) Expression of CD45RA and CD45RO on PKH26– cells (originally CD45RA+) after activation and transduction. Representative dot plots (E) and summary of 3 experiments (F). (G) CD45RA+ cells were labeled with PKH26, and transduction on PKH26- cells (originally CD45RO+) was assessed (n = 3).

IL-7 and IL-15 do not promote preferential transduction of naïve T cells. (A) Schematic representation of the labeling process to distinguish T cells originated from naïve (CD45RA+) and antigen-experienced (CD45RO+) T cells by labeling with PKH26 dye before activation and retroviral transduction with either IL-2 or IL-7 and IL-15. (B) Gating strategy and representative data showing CAR expression by T cells originated from naive T cells. (C) CAR expression in PKH26– cells (originally CD45RA+) after activation and transduction (n = 5). (D) Percentage of Ki67+ cells on PKH26– cells (originally CD45RA+) prior to retroviral transduction (n = 5). (E-F) Expression of CD45RA and CD45RO on PKH26– cells (originally CD45RA+) after activation and transduction. Representative dot plots (E) and summary of 3 experiments (F). (G) CD45RA+ cells were labeled with PKH26, and transduction on PKH26- cells (originally CD45RO+) was assessed (n = 3).

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