CFD simulations and in vivo occlusive thrombosis. (A) Blood velocity map inside the MCA is represented at different stages of occlusive thrombus formation. (B) Computed blood flow in the MCA from 5% to 95% of thrombus formation. (C) Peak shear rate at the thrombus surface during thrombosis. (D) Profile of the shear rate along the thrombus surface at different stages of thrombus formation. (E) Schematic drawing of the 3 different regions in occlusive thrombi. (F) Representative immunohistological images of the MCA 20 minutes after topical application of 10% FeCl₃ (representative of 5 mice). (G) Representative laser Doppler profile of the CBF inside the MCA territory following 10% FeCl₃ application and treatment by either saline or GR (10 mg/kg, a GpIIb/IIIa inhibitor) 20 minutes after thrombosis. (H) Corresponding quantification of the CBF at the end of the monitoring period (40 minutes posttreatment, n = 5). (I-K) Same as in F-H but using a solution of 20% FeCl₃. ns, not significant.