Inhibition of GpIbα-VWF interactions improves stroke outcome after occlusive thrombosis. (A) Representative laser Doppler profiles of mice treated with saline, ATA, or GR 20 minutes after occlusive thrombus formation. (B) Mean CBF (as assessed by laser Doppler flowmetry 60 min after MCAo) in brains of mice treated with saline, ATA, or GR 20 minutes after MCAo (n = 10-15 mice/group). (C) Corresponding mean ischemic lesion sizes at 24 h post-MCAo (n = 10-15 mice/group). (D) Representative laser Doppler profiles of brains of mice treated with Fab from control IgG, Xia.B2 (2.5 mg/kg, anti-GpIbα), or Leo.H4 (2.5 mg/kg, anti-GpIIb/IIIa) after thrombus formation. (E) Corresponding mean CBF (as assessed by laser Doppler flowmetry 60 minutes after MCAo; n = 6 mice/group). (F) Representative T2-weighted images of Fab from control IgG-, Xia.B2-, and Leo.H4-treated mice 24 hours after FeCl3 application (quantification of lesion sizes is presented in G, n = 6 mice/group). ns, not significant.
