The role of Gα12 and Gαq in vWF secretion and primary hemostasis. (A) vWF levels in plasma from WT vs Gα12^−/−, Gα11^−/−, EC-Gαq^−/−;Gα11^−/−, and EC-Gα13^−/−;Gα12^−/− mice. *P < .05 vs WT; n = 6/group. (B) Representative vWF multimer gel from WT, Gα12^−/−, and Gα11^−/− mouse plasma. Quantitative multimer analysis shows no difference between Gα12^−/− (n = 3) and WT or Gα11^−/− mouse vWF. (C) Constitutive and PAR-1 agonist peptide-induced vWF release from WT, Gα12^−/−, and EC-Gαq/;Gα11^−/− isolated perfused mouse lungs. Time-course of basal and PAR-1-specific peptide TFLLR-evoked vWF release into mouse lung perfusate. (D) vWF in WT, Gα12^−/− and EC-Gαq^−/−;Gα11^−/− mouse lung extracts from untreated lungs or after PAR-1 peptide stimulation. *P < .05 vs WT + TFLLR; n = 6/group. See also supplemental Figure 1B.