Figure 3
Figure 3. Runx1bEx6− and Runx1bEx6e have lower transactivation efficiency than full-length Runx1bEx6+. 293T cells were transfected in duplicate with the indicated construct together with CBFβ expression construct, M-CSF receptor promoter-firefly luciferase reporter construct and Renilla luciferase reporter construct as a transfection efficiency control. (A) Transfection with single Runx1 isoform. Runx1bEx6− shows lower transactivation than Runx1bEx6+. Runx1bEx6e has no transactivation. Values were normalized to Renilla luciferase signal, and promoter activity of MIP-transfected cells was set to 1. Representative result of 2 independent experiments is shown. (B) Cotransfection of MIP, Runx1bEx6−, or Runx1bEx6e with Runx1bEx6+. Runx1bEx6− and Runx1bEx6e show additive and synergistic effect, respectively. Values were normalized to Renilla signal, and MIP was set to 1. Representative result of 2 independent experiments is shown.

Runx1bEx6−and Runx1bEx6e have lower transactivation efficiency than full-length Runx1bEx6+. 293T cells were transfected in duplicate with the indicated construct together with CBFβ expression construct, M-CSF receptor promoter-firefly luciferase reporter construct and Renilla luciferase reporter construct as a transfection efficiency control. (A) Transfection with single Runx1 isoform. Runx1bEx6− shows lower transactivation than Runx1bEx6+. Runx1bEx6e has no transactivation. Values were normalized to Renilla luciferase signal, and promoter activity of MIP-transfected cells was set to 1. Representative result of 2 independent experiments is shown. (B) Cotransfection of MIP, Runx1bEx6−, or Runx1bEx6e with Runx1bEx6+. Runx1bEx6− and Runx1bEx6e show additive and synergistic effect, respectively. Values were normalized to Renilla signal, and MIP was set to 1. Representative result of 2 independent experiments is shown.

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