Evidence of neovascularization after implanting autologous BOECs mixed in various fibrin-based matricies into the omentum. (A) Autologous BOECs isolated from 2 normal dogs (Normal 1 and Normal 2) were implanted into 6 individual sites (sites #1 to #6) in the greater omentum in each of the dogs. Various numbers of BOECs (2.5 × 106 to 4 × 107) were implanted in each site. The fibrin-based matrix consisted of fibrinogen (either autologous or obtained from the Beriplast kit) and human thrombin from the Beriplast kit. Growth factors were included with the cells implanted into sites #2, #4, and #6. (B) Normal dog #2 was biopsied 3 months after implanting the autologous BOECs. Hematoxylin and eosin staining of a representative sample isolated from site #6. Arrows indicate areas of neovascularization. (C) Confocal microscopic analysis of a biopsy sample taken from site #6 and immunostained for FVIII (red). (D-F) Representative images of one of the structures appearing to be a small blood vessel immunostained with antibodies for FVIII (red), vWF (green), and DAPI (blue).
