Genetic variants and frequencies of secondary CMML cases with sequential samples. Cases 1 to 7 (A-G, respectively) were sequentially sequenced cases at ≥1 time point of MDS and 1 time point of CMML. Although previously frozen or fresh bone marrow mononuclear cells (BMNCs) and peripheral mononuclear cells (PBMCs) were included in this analysis, sequential samples were only analyzed if they were isolated from the same source. Genes are color coded and VAFs are annotated for each case. The gene sequenced with our targeted gene panel were as follows: ASXL, CBL, CEBPA, DNMT3A, ETV6, EZH2, FLT3, IDH1, IDH2, JAK2, KIT, KRAS, MLL, NPM1, NRAS, RUNX1, SETBP1, SF3B1, SRSF2, TET2, TP53, U2AF1, WT1, ZRSR2, MPL, and ABL1. To be a true mutation, variants must have resulted in a frame shift or nonsense mutation. Missense mutations were only included if they had been previously reported in hematologic malignancies and confirmed to be somatic in the literature.