Analysis of the procoagulant and proabortive effect induced by MBB2 in complement-deficient or complement-depleted animals. (A) Thrombus formation and (B) vessel occlusion were monitored by intravital microscopy at different time intervals in LPS-primed C6^−/− and wild-type (WT) PVG rats receiving MBB2. Percentage of (C) fetal loss and (D) fetal weight in normal and C5-depleted mice treated with MBB2. Immunofluorescence analysis of (E) rat mesenteric tissue and (F) fetal implantation sites in normal and complement-deficient animals treated with MBB2 for the deposition of scFv-Fc, C3, and C9. Scale bars equal 50 μm. The procoagulant and the proabortive effects of MBB2 were evaluated and analyzed as described in Figure 2. The data of vessels occlusion and fetal weight are expressed as mean ± SD; percentages of fetal loss are expressed as median ± interquartile range presented as box plots. *P < .05, **P < .01.