Effect on the pumping rate of LC with different organ tropism, purified from different patients’ urine and serum or obtained as recombinant. Effect of (A) BJ LC (100 µg/mL) purified from 8 patients (3 heart AL, 1 kidney AL, 1 soft tissue AL, 3 MM), (B) serum-free LC (50 µg/mL) purified from 11 patients (5 heart AL, 4 kidney AL, 2 MM), and (C) recombinant LC (100 µg/mL) obtained from 4 patients (3 heart AL and 1 kidney AL) on pharyngeal pumping. Nematodes (100 worms/100 µL) were incubated for 2 hours, in the absence of OP50 E coli, with different amyloidogenic noncardiotoxic or cardiotoxic LCs or nonamyloidogenic LCs from patients with MM. The pharyngeal pumping rate was scored 20 hours after plating the worms on NGM agar plates seeded with fresh OP50 E coli as food. Control worms received vehicle alone (Vehicle). Each dot on the scatterplot represents the mean value of pumps per minute obtained for each single protein from 3 independent assays (n = 30 worms/assay). These values were used to calculate the mean ± SE for LCs with the similar organ tropism (horizontal line) and to perform a statistical comparison across the different groups. **P < .001 vs vehicle, °P < .05 and °°P < .01 noncardiotoxic vs cardiotoxic LCs, according to 1-way ANOVA followed by Bonferroni post hoc test.