F(ab′)2 fragments of 34.1.2s induce MIP-2 production and neutrophil accumulation, but not hypothermia, edema, or mortality in SCID mice. Time course of (A) rectal temperatures and (B) survival of SCID mice either not injected (○) or injected with 50 µg 34.1.2s (□), 75 µg F(ab′)2 fragments (♢), or 75 µg Fc fragments (△). Two hours after injection of the indicated proteins, the mice were euthanized, (C) serum MIP-2 levels were measured, (D) wet:dry lung weight measurements were made, and (E) the percentage of pulmonary neutrophils was measured. Data are expressed as means ± SD of the indicated parameters (y-axes) in A and C-E and as means in B (n = 6 to 21 mice in each group). The ★ indicates significance (P < .05) between mouse groups and was determined by using one-way ANOVA. All other comparisons were not significant except for control vs Fc (C, P < .05) and control vs F(ab′)2 and Fc (E, P < .05).
