Siglec-G deficiency enhances DAMP-induced inflammatory responses from hematopoietic APCs. (A-B) Both B6 and Siglec-G^−/− BMDCs, (C) peritoneal macrophages, and the GI tract DCs were harvested and stimulated with HMGB-1 (5 μg/ml) for 16 hours. The supernatants were analyzed for TNF-α (A,C-D), and IL-6 (B). (E) TLR-4 expression on BMDCs. The data are representative of 3 independent experiments and show the mean ± SEM.