![Figure 2. The liver-specific KO mice recapitulate the phenotype of the total KO mice. (A) FPN and ferritin expression in the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates by western blotting. (B) Immunohistochemistry for FPN in the duodenum of Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 50 μm. (40/0.60, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (C) Western blotting in duodenum scrapings of 6-month-old Hepc∆total, Hepc∆liver, and control mice. (D) Liver, pancreas, and plasma iron in 2-, 3-, and 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates. n > 5. *P < .05, ***P < .001. ns indicates not significant. Perls’s blue staining of the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 100 μm. (20/0.4, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (E) Plasma hepcidin in 6-month-old control, Hepc∆total, and Hepc∆liver mice. n = 4.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/23/10.1182_blood-2014-01-550467/4/3646f2.jpeg?Expires=1735566763&Signature=aaQ1DuOUdkGbdNdw6Y1J2dKsRMswHw7CAK25EeibHdWc0wUaJP4IYAXNpn~dj~WJYB1ZtJGKzWaqFi96JGlOBN8xecdV9ISkdLSrJJ9L9XNR22WWuAO1~0en1492AcE3udm~gLrclkHMO7J5tDY~2fZcMM6zQDdUPpNxyNUl2diums5mZ1u3CQ-8NDIk7kBem6HRMhEfUvzBYHv9DsLLN~N1J-bwCakck7HEq459zzGFq5StQWuqRdXxDi9MqSxxmTVAM9Vnm73kxdfcK6PhW3v8VgrcC8GMHDwj-a-VX3vmzzRYMDogp78ObTyoMIVytTM8gyw8rDh5tWpgBavw9g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The liver-specific KO mice recapitulate the phenotype of the total KO mice. (A) FPN and ferritin expression in the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates by western blotting. (B) Immunohistochemistry for FPN in the duodenum of Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 50 μm. (40/0.60, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (C) Western blotting in duodenum scrapings of 6-month-old Hepc∆total, Hepc∆liver, and control mice. (D) Liver, pancreas, and plasma iron in 2-, 3-, and 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates. n > 5. *P < .05, ***P < .001. ns indicates not significant. Perls’s blue staining of the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 100 μm. (20/0.4, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (E) Plasma hepcidin in 6-month-old control, Hepc∆total, and Hepc∆liver mice. n = 4.
