CMV-specific CD8⁺T cells from CLL patients exhibit decreased expression of the exhaustion markers PD1, CD160, and CD244 as compared with those from HCs. (A) Percentages and geometric MFI of CD160, CD244, and PD1 within CMV-specific CD8⁺ T cells using CMV tetramers in untreated CLL patients (n = 18) and age-matched HCs (n = 7) were measured by flow cytometry. (B) Geometric MFI of CD160, CD244, and PD1 within CMV-specific CD8⁺ T cells, stratified according to different clinical parameters. (C) Percentages of CD160, CD244, and PD1 within total CD8⁺ T cells and absolute numbers of CD160, CD244, and PD1 in total CD8⁺ T cells from CLL patients (n = 38) and age-matched HCs (n = 13). For panels A to C, horizontal bars, boxes, whiskers, and dots represent median, 25%/75% quartiles, range, and outliers, respectively. Significant differences in the expression of different markers between CLL and HCs are presented as *.01 ≤ P < .05; **.001 ≤ P < .01; ***P < .001 (Mann-Whitney U test).