Knockdown of aggf1 expression results in abnormal definitive hematopoiesis during zebrafish embryogenesis. Knockdown of aggf1 expression reduces the runx1 signal (A-D) or c-myb signal (E-H) in the ventral wall of the dorsal aorta, 2 independent markers for HSCs. Zebrafish embryos (1- to 2-cell stage) were injected with 4 ng of Std-MO (A,E), 2 ng of aggf1 MO1 (B,F), 4 ng of aggf1 MO2 (C,G), or 2 ng of aggf1 MO1 together with 100 pg of aggf1 mRNA (D,H) and used for WISH at 30 hpf with an antisense probe for runx1 or c-myb. Knockdown of aggf1 expression abolishes the rag1 signal, a marker for lymphoid cells, at 5 dpf (I-K). (A-H) Embryos are shown in a lateral view with the anterior on the left. (I-K) Embryos are shown as a dorsal view with the anterior at the top.