Specification of hemangioblasts and hematopoietic cells is defective in aggf1 morphants. (A-C) Zebrafish embryos injected with Std-MO (2 ng; A,D,G), aggf1 MO1 (2 ng; B,E,H), or MO1 (2 ng) together with 100 pg of aggf1 mRNA (C,F,I), and used for WISH with probes fli1 and scl (markers for hemangioblasts) and gata2 (marker for hematopoietic cells) at 8 somites. Expression of scl, fli1, and gata2 is severely reduced in the posterior lateral plate mesoderm (PLPM) by knockdown of aggf1 expression. Embryos are shown at a dorsal view with the anterior at the top.