Overexpression of scl rescues defects in hematopoiesis in aggf1 morphants. Zebrafish embryos were injected with Std-MO (4 ng; A,D), aggf1 MO2 (4 ng; B,E), or aggf1 MO2 (4 ng) together with in vitro synthesized full-length scl mRNA (75 pg) (C,F) and used for WISH at 8 somites with antisense probes for pu.1 (A-C) and gata1 (D-F). Reduced expression of pu.1 (marker for myeloid progenitor) in anterior lateral plate mesoderm by knockdown of aggf1 expression was rescued by overexpression of scl mRNA (A-C). Coinjection of scl mRNA rescued decreased expression of gata1 (marker for erythroid progenitor) in PLPM caused by aggf1 MO2 (D-F). (A-C) Anterior view with the ventral side at the top. (D-F) Dorsal view with the anterior at the top.