![Figure 1. FP-positive CEL with concomitant KIT D816V mutation. (A) Hypercellular BM biopsy with a marked increase in eosinophils (Giemsa stain). (B-E) Phenotypically aberrant MCs are arranged in loose clusters and as interstial infiltrates (B) tryptase, (C) CD117, (D) CD2, and (E) CD25. (F-I) fluorescence in situ hybridization with a 4q12 tri-color rearrangement probe (F) alone or (G-H) combined with tryptase IH (fiction) (note nuclei with green/aqua fusion signals [arrows] as a surrogate for CHIC2 deletion). Normal tricolor green/orange/aqua fusion signals are marked by an arrowhead. (J) c-kit antisense sequence of microdissected MCs from FIP1L1-PDGFRA–positive CEL showing a KIT D816V mutation. (A-E) Photomicrographs by Horn Imaging Camera (Aalen, Germany) adopted to a Zeiss Imager.M1 (Carl Zeiss, Oberkochen, Germany) microscope. (F-I) Imaging system ApoTome.2 (Carl Zeiss).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/4/10.1182_blood-2013-10-530642/4/595f1.jpeg?Expires=1735500749&Signature=DAA6-pCIc4GLPVxYRvI0uTEgcqZ5Ho2~HeBHnTXhRe0IlAzloQfClCB90qONP4Chzrcx10GQsa8ZpHsBkxSqSQXcIQQsOxd1plxtIgTeQL1JcjlbRrEWUkM389N6toxuDMdwbVTgvM4VbJDEZZtSDHLZpKOaugvYKGmmhDOn7X8LR03MGZ22OuHhztYQPbJY9Ip3R~FNJ-7aFf~ZQQNCyc4ZdDZ4pReUCO~nrjTJ-VrnUl0lTkOSli2UZYSKAJThtilUc0M9uwFE2ibZYWAUKz4BaGxqY2gQyQW7~yMsKY0NZU6vAV9WEPZ2FfbHQ8qG27b0pwI3JB77F4ifj1QaIg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
FP-positive CEL with concomitant KIT D816V mutation. (A) Hypercellular BM biopsy with a marked increase in eosinophils (Giemsa stain). (B-E) Phenotypically aberrant MCs are arranged in loose clusters and as interstial infiltrates (B) tryptase, (C) CD117, (D) CD2, and (E) CD25. (F-I) fluorescence in situ hybridization with a 4q12 tri-color rearrangement probe (F) alone or (G-H) combined with tryptase IH (fiction) (note nuclei with green/aqua fusion signals [arrows] as a surrogate for CHIC2 deletion). Normal tricolor green/orange/aqua fusion signals are marked by an arrowhead. (J) c-kit antisense sequence of microdissected MCs from FIP1L1-PDGFRA–positive CEL showing a KIT D816V mutation. (A-E) Photomicrographs by Horn Imaging Camera (Aalen, Germany) adopted to a Zeiss Imager.M1 (Carl Zeiss, Oberkochen, Germany) microscope. (F-I) Imaging system ApoTome.2 (Carl Zeiss).
