Gadd45a deficiency enhances HSC proliferation after 5-FU treatment. (A) The representative FACS plots of the LSK population at the indicated time points after 5-FU treatment. (B) The dynamic change of absolute number of LSK cells after 5-FU injection detected by flow cytometry (n = 3-5). (C) The representative FACS plots and absolute number of CD34^-LSK cells in BM at day 21 after 5-FU injection are shown (G^+/+, n = 5; G^−/−, n = 3). (D-E) BrdU was delivered by intraperitoneal injection 1 hour before euthanasia at day 12 after 5-FU injection. The representative FACS plots and percentage of S or G2M phases in LSK cells and HPCs are shown (G^+/+, n = 4; G^−/−, n = 5) (*P < .05, **P < .01). N.S., not significant.