JAM-A recruits Csk to the integrin complex. (A) Representative anti-Csk immunoblots of proteins immunoprecipitated with anti-α_IIb or β₃ or isotype-specific IgG (cIgG) from lysates of Jam-A^wt/wt and Jam-A^gt/gt mouse platelets. Blots were reprobed with anti-β₃ to ensure equal loading. Note: The mobility of the Csk band is the same as the IgG heavy chain. Therefore, light chain-specific horseradish peroxidase-conjugated secondary antibody was used to detect Csk. (B,C) Quantitation of normalized band intensities of “A” anti-β₃ (B; P = .003) and anti-α_IIb (C; P = .03) from 3 independent experiments.