Gel filtration to assess interactions of C5b,6 and C7 with polyP. The effect of polyP on the gel filtration elution profiles of C5b,6 and C7 was evaluated. (A) The presence of polyP_>1000 (10 mM) does not affect the retention time or elution profile of C7. (B) P₁ (monoP) (10 mM) does not affect the retention time or elution profile of C5b,6. However, the presence of polyP (10 mM) binds to and shifts C5b,6 to a more highly oligomerized or aggregated state.