Interaction of PBMC subpopulations with EVs. Monocytes and T cells were purified from PBMCs and stained with PKH67 green membrane dye. EVs were stained with PKH26 red membrane dye. EVs were added to monocytes or T cells and incubated in a 5% CO₂ incubator for 3 or 24 hours. Cells were fixed with 2% PFA and subject to fluorescence microscopy or flow cytometry. Data are representative of 3 independent experiments. (A) Monocytes 3 hours after incubation with EVs. (B) Monocytes 24 hours after incubation with EVs. (C) T cells 3 hours after incubation with EVs. (D) T cells 24 hours after incubation with EVs.