Effect of methylation (<20% vs ≥20%) on OS from time of diagnosis in subgroups of patients segregated by IGHV mutational status, CD38 expression, and ZAP-70 protein expression. (A) Methylation levels tend to segregate patients who are M-IGHV (P = .03) or U-IGHV (P = .21). (B) Methylation levels tend to segregate patients who are CD38 negative (P < .0001) or CD38 positive (P = .11). (C) Methylation levels tend to segregate patients who are ZAP-70 negative (P = .006) or ZAP-70 positive (P = .19).