CD123⁺ and CD123^– CD34⁺/CD38^lo CP-CML cells show self-renewal capacity. FACS-sorted subpopulations of CP-CML early progenitor and stem cells were tested for their colony-forming and replating ability in methylcellulose media (A) as well as their long-term culture initiating potential (B). Graphs in both panels show an average of 5 individual patient samples. (C) The percentage of Ph⁺ cells within FACS-sorted populations and of plucked primary and secondary CFU and LTC-IC colonies was determined by BCR-ABL1 FISH (n = 3, *P < .05 by unpaired Student t test).