Analysis of longer chain polyP-700-mediated proinflammatory signaling effects of HMGB1 and H4 in the permeability assay. (A) Endothelial cell permeability in response to increasing concentrations of polyP-700 (0.5-10 μM) was measured. (B) Endothelial cell permeability in response to HMGB1 (10 nM) was measured in the presence of increasing concentrations of polyP-700 (0.035-0.5 μM) was measured. (C) Endothelial cell permeability in response to H4 (0.44 μM) was measured in the presence of increasing concentrations of polyP-700 (0.035-0.5 μM). (D) Endothelial cell permeability in response to increasing concentrations of HMGB1 (1.25-10 nM) was measured in the presence of a fixed concentration of polyP-700 (0.067 μM). (E) Endothelial cell permeability in response to increasing concentrations of H4 (44-440 nM) was measured in the presence of a fixed concentration of polyP-700 (0.067 μM). *P < .05; **P < .01; ***P < .001.