Requirement for CDK6 in MLL-rearranged hematopoietic cells. (A) Effects of CDK6 suppression in MLL-AF9^pos and MLL-AF9^neg AML cell lines. (B) Effects of CDK6 suppression in MLL-AF4^pos MV4-11 and MLL-AF6^pos ML-2 cells. (C) Rescue of viable cell number by expression of the CDK6 coding sequence in NOMO-1 cells transduced with an shRNA targeting the CDK6 3′ UTR. (D) CDK6 protein expression of cells used in panel C. (E) Effects of Cdk6 suppression in suspension cultures of Ba/F3 cells and murine HSPC transduced with MLL-AF9. (F) Effects of Cdk6 suppression in methylcellulose cultures of murine HSPC transduced with MLL-AF9 or MOZ-TIF2. Original magnification, ×25.