Western blot validation of increased Src Tyr530 phosphorylation. Washed human platelets (3 × 108/mL) were incubated with iloprost (5nM) for the indicated time and processed for western blot analysis with antibodies directed against P-SrcTyr530 and total Src. Signals were quantified as described in the Figure 1B legend. Shown are representatives of 3 independent experiments.