Mutated FLT3 regulates the activity of SIRT1 via the ATM-DBC1 axis. (A) MV4-11 and Molm-14 cells were treated with PKC412 for 4 hours, as indicated, and coimmunoprecipitation was performed using an anti-DBC1 antibody. (B) Quantitative RQ-PCR analysis of p21, BAX, Puma, and Noxa expression on treatment of MV4-11 cells with PKC412 for 4 hours. Expression of target genes was normalized to GAPDH. Shown is the fold increase compared with untreated control of 3 independent experiments. (C-D) Western blots of MV4-11 and Molm-14 cells treated with PKC412 for 24 hours, as indicated (C), or with 100 nM PKC412, irradiation (2 Gy; IR), or in combination (D) probed with the indicated antibodies. (E-F) Coimmunoprecipitation using an anti-DBC1-antibody was performed in MV4-11 cells treated with 100 nM PKC412, irradiation (2 Gy; IR), or in combination for 4 hours. Immunoblots were probed as indicated.