Decreasing Id2 levels in Gfi-1^−/− BMCs restores c-Kit^hi LK subpopulation in short-term BM reconstitution. (A) Flow cytometric analysis of donor LSK and LK c-Kit^hi/c-Kit^lo populations 36 days after BM transplantation. LK cells were divided into c-Kit^hi and c-Kit^lo subpopulations. Lack of donor c-Kit^hi LK cells in Gfi-1^−/− BMCs (middle) was restored in Gfi-1^−/−;Id2^+/− BMCs (right). (B) Bar graph showing proportion of CMP, MEP, and GMP donor cells in c-Kit^hi and c-Kit^lo cells, as determined by flow cytometry. Results are representative of 3 independent experiments. (C) Bar graph showing total numbers of donor LSK LT-HSCs, ST-HSCs, and MPPs in recipient BMCs. Cellularity was calculated based on total BMCs isolated from 2 femurs and 2 tibias of each recipient. These data are representative of 3 independent experiments. Data are presented as the mean ± SD. *P < .05 for Gfi-1^−/− compared with Gfi-1^−/−;Id2^+/− ST-HSC cellularity. (D) Flow cytometric analyses show the proportion of CMPs, MEPs, and GMPs found in total LK (top), LK c-Kit^hi (middle), and LK c-Kit^lo (bottom) of wild-type mouse BMCs.