G6PD expression and enzymatic activity in cells from patients with G6PD deficiency (def) in response to HDACi. (A) G6PD mRNA levels in WT and G6PD-Brighton B-cell lines after treatment with 3 mM NaBu over 36 hours. (B) G6PD protein levels assessed by western blotting (top; representative of 1 of 3 independent experiments is shown) and quantified (n = 3; bottom). (C) G6PD enzymatic activity assessment at the corresponding time points.