Figure 3
Figure 3. fos acts downstream of ncor2 to regulate HSC development. (A) The expression level of runx1 and cmyb in embryos injected with fos mRNA at 24 and 36 hpf. (B) qPCR analysis of runx1 expression within embryos injected with a series of doses of fos mRNA (mean ± SD, n = 3, *P < .05). (C) WISH result showed the expression level of runx1 or cmyb in the AGM region at 36 hpf (top and middle panels). The HSCs number in the control, ncor2 MO injected, ncor2 and fos MOs coinjected embryos at 40 hpf, within the kdrl:mCherry/cmyb:GFP line (bottom panel). White arrows indicate HSCs in the AGM region. (D) The quantification of kdrl+/cmyb+ HSCs shown in (C) (mean ± SEM, n = 13, *P < .05). (E) Western blot demonstrated protein level of Runx1 in the control, ncor2 MO injected, ncor2 and fos MOs coinjected embryos at 36 hpf.

fos acts downstream of ncor2 to regulate HSC development. (A) The expression level of runx1 and cmyb in embryos injected with fos mRNA at 24 and 36 hpf. (B) qPCR analysis of runx1 expression within embryos injected with a series of doses of fos mRNA (mean ± SD, n = 3, *P < .05). (C) WISH result showed the expression level of runx1 or cmyb in the AGM region at 36 hpf (top and middle panels). The HSCs number in the control, ncor2 MO injected, ncor2 and fos MOs coinjected embryos at 40 hpf, within the kdrl:mCherry/cmyb:GFP line (bottom panel). White arrows indicate HSCs in the AGM region. (D) The quantification of kdrl+/cmyb+ HSCs shown in (C) (mean ± SEM, n = 13, *P < .05). (E) Western blot demonstrated protein level of Runx1 in the control, ncor2 MO injected, ncor2 and fos MOs coinjected embryos at 36 hpf.

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