vegfd functions downstream of nocr2 and fos to repress HSC emergence. (A) The expression pattern of vegfd in the control, ncor2 morphants, and fos overexpressed embryos at 26 hpf. Red arrows mark the AGM region, whereas white arrow marks the tail bud. (B) qPCR result showed the expression level of vegfd in the control, ncor2 morphants, and fos-overexpressed embryos at 26 hpf (mean ± SD, n = 3, *P < .05). (C) The expression pattern of runx1 and cmyb in the control and vegfd-overexpressed embryos at 36 hpf (top and middle panels). The expression level of runx1 in the control or embryos coinjected with the fli1-ep-vegfd-gfp plasmid and tol2 mRNA (bottom panel). (D) The expression pattern of dll4 and ephrinB2a in the control and vegfd-overexpressed embryos. (E) Knocking down vegfd could partially rescue the decrease of runx1 in ncor2 morphants.