Identification of miR-150 targets by gene expression analysis. (A) Microarray data for protein-coding gene expression (Affymetrix HG-U133+2 GeneChips) were compared in CLL samples stratified based on low (n = 32) vs high (n = 32) levels of miR-150. The samples from the lowest and highest tercile were compared (SAM in MeV, fold change >1.5, false discovery rate <0.1). This analysis identified differential expression of 58 genes (72 probes) between CLL cells expressing low vs high levels of miR-150. GAB1 and FOXP1 are marked with a black arrow. (B) The evolutionary conserved binding sides for miR-150 in GAB1 and FOXP1 3′UTR (TargetScan). (C-E) The association between miR-150 levels and the expression of mRNAs for GAB1 (C), FOXP1 (D), or c-MYB (E) in a validation cohort of 60 CLL samples. Each point represents the average value for gene expression from 10 CLL samples. The standard deviation about the mean is indicated by error bars.