Delivery of FVIII antigen to the GALT and into circulation. (A-C) Immunostains (original magnification, ×200) of ileum cryosections from unfed (A, negative control) or CTB-C2-fed (B, lamina propria; C, Peyer’s patch) BALB/c mice with hemophilia A. Stains are for C2 domain of FVIII (green), CD11c (red), and nuclei (DAPI; blue). (D) Human FVIII antigen levels were measured in plasma or liver protein extract of the CTB-HC-fed C57BL6/129 and BALB/c mice with hemophilia A and WT-fed control mice of the same strain, using HC-specific ELISA. All data are shown for individual mice and as averages ± SEM.