Bacterial adhesion to activated endothelium in vivo is VWF and vWbp -mediated. (A) In vivo venous mesenteric perfusion model with C57Bl/6-Vwf^+/+ and C57Bl/6-Vwf^−/− mice. A total of 5 µL of the Ca²⁺-ionophore A23187 (10 mM) was applied to the region of the visualized vascular bed to trigger EC activation and VWF release. A suspension of carboxy-fluorescein–labeled WT, vwb, or coa/vwb strains was injected through the jugular catheter. Where indicated, bacterial inoculation was preceded by a bolus of 50 µL of dabigatran (10 µM). All results are expressed as mean ± SEM. **P < .01, ***P < .001, n ≥ 7. (B) Image of in vivo venous mesenteric perfusion model with C57Bl/6-Vwf^+/+ mice.