Figure 3
Shear stress enhances the production of PPTs and PLPs. PLPs generated under shear flow display enhanced in vitro functional activity. (A) A mature and polyploid Mk displays extensive PPTs in static culture at day 12. Scale bar: 50 µm. (B-D) Mks at day 12 were exposed to shear flow at 1 dyn/cm2 for 2 hours or 4.0 dyn/cm2 for 0.5 hour. Both adherent and nonadherent cell fragments were analyzed post shear exposure. Numbers (B) of PLPs and PPTs per slide post shear exposure vs static control. Two functionality assays, CD62P exposure (C) and fibrinogen binding (D), were performed on the harvested PLPs and demonstrate enhanced activity for PLPs generated under shear flow. Error bars indicate SEM of 3∼4 biological replicates. **P < .01.
