Variant allele frequencies in selected genes. (A) Quantitative measure of variant-containing reads estimates the abundance of these mutations (uncorrected for allele copy number). Mutations of TET2 (green), TP53 (orange), and splicing factor genes (red) are often present in the dominant clone, whereas mutations of tyrosine kinase–signaling genes (blue) are often present in smaller clones. Mutations of ASXL1 (yellow) are more widely distributed. (B) Analysis of samples with both TET2 and ASXL1 mutations indicate that ASXL1 mutations are most often codominant with, or smaller than, TET2-mutant clones.